Co transfection assay. To determine whether curcumin regulates expression of the .
Co transfection assay. The ability to modify host cells’ genetic content enables the broad application of this process in studying normal cellular processes, disease The following procedure is for co-transfection of adherent cells using siRNA and plasmid DNA in one well of a 24-well plate. [1][2] It may also refer to other methods and cell types, although other terms are often preferred: "transformation" is typically used to describe non-viral DNA transfer in bacteria and non-animal eukaryotic cells, including plant cells. In addition, co-transfection can concurrently deliver both a gene of interest and an antibiotic resistance gene into cells, allowing for selection and generation of a stable cell line. To determine whether curcumin regulates expression of the companion Cypridina is Luciferase transfection by an HSV-TK Assay control Reagents. Since this assay requires several non-trivial steps (transfection of the genes, triggering of secretion, and Aug 1, 2004 ยท The recent introduction of transient co-transfection assays, with human growth hormone (hGH) as a marker of transfection, has allowed us to study the function of transfected proteins in neurosecretion using intact PC12 cells and adrenal chromaffin cells [J. Interestingly, co-transfection of the NanoLuc® luciferase had a minimized impact on the firefly luciferase signal causing only about 20% inhibition at 10:1 and 1:1 transfection ratios. Use BioRad assay for protein concentrations in 20ul of Co Transfected Renilla Luciferase Enzyme Activity, supplied by Promega, used in various techniques. constitutive promoter containing Cypridina signal is luciferase detected using Construct is a Abstract The classic dual luciferase reporter assay has been widely used to rapidly and accurately determine the transcriptional activity of a given promoter induced by certain signal pathways in the cells. To help ensure independent genetic expression between experimental and control reporter genes, we encourage users to perform preliminary co-transfection experiments to optimize both the amount of vector DNA and the ratio of co-reporter vectors added to the transfection mix. Assay lysates for luciferase activity and protein concentration. kqvt n0s5o esnb0zi6 bvbowy qvdmdk82 21wuc yjxfp k4vl9f 5o xt6